Mouse Cytokine Array C6

    


RayBio® C-Series Mouse Cytokine Antibody Array 6 Kit. Detects 97 Mouse Cytokines. Suitable for all liquid sample types.



 AAM-CYT-6-2

Antigen Information

Gene Symbols:
AREG | AXL | CCL11 | CCL17 | CCL19 | CCL2 | CCL20 | CCL21 | CCL22 | CCL24   View more

Assay Format

Number of Targets Detected:
97
Species Detected:

  • Mouse

Compatible Sample Types:

  • Cell Culture Supernatants

  • Cell Lysates

  • Plasma

  • Serum

  • Tissue Lysates

Design Principle:

  • Sandwich-based

Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use

  • No specialized equipment needed

  • Compatible with nearly any liquid sample

  • Proven technology (many publications)

  • Highly sensitive (pg/ml)

  • Sandwich ELISA specificity

  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
6Ckine
(CCL21)
ALK-1
Amphiregulin
Axl
BLC
(CXCL13)
Cardiotrophin-1
(CT-1)
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD30
(TNFRSF8)
CD30 Ligand
(TNFSF8)
CD36
(SR-B3)
CTLA-4
(CD152)
CXCL16
Decorin
DKK-1
E-Cadherin
EGF
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Epigen
E-Selectin
Fas Ligand
(TNFSF6)
Fc gamma RIIB
(CD32b)
Flt-3 Ligand
Fractalkine
(CX3CL1)
Galectin-1
Gas 6
GCSF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
Granzyme B
HAI-1
HGF
IFN-gamma
IGFBP-5
IGFBP-6
IGF-2
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R alpha
IL-3
IL-4
IL-5
IL-6
IL-9
IL-10
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-15
IL-17A
IL-17 RB
IL-17E
(IL-25)
IL-17F
IL-20
IL-21
I-TAC
(CXCL11)
JAM-A
(CD321/F11R)
KC
(CXCL1)
Leptin
Leptin R
L-Selectin
(CD62L)
Lungkine
(CXCL15)
MAdCAM-1
MCP-1
(CCL2)
MDC
(CCL22)
MFG-E8
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 gamma
MIP-2
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MMP-2
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
Prolactin
Pro-MMP-9
RANTES
(CCL5)
SCF
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TACI
(TNFRSF13B)
TARC
(CCL17)
TNF alpha
Thrombopoietin
(TPO)
TRANCE
(TNFSF11)
TROY
(TNFRSF19)
TWEAK R
(TNFRSF12)
VCAM-1
(CD106)
VEGF-A
VEGFR1
VEGFR3
VEGF-D



Species Detected

Mouse

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components

  • Mouse Cytokine Antibody Array C6 Membranes

  • Blocking Buffer

  • Wash Buffer 1

  • Wash Buffer 2

  • Biotinylated Detection Antibody Cocktail

  • Streptavidin-Conjugated HRP

  • Detection Buffer C

  • Detection Buffer D

  • Lysis Buffer

  • 4-Well Incubation Tray

  • Plastic Sheets

  • Array Templates

  • Manual

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables

  • Orbital shaker or oscillating rocker

  • Tissue Paper, blotting paper or chromatography paper

  • Adhesive tape or Saran Wrap

  • Distilled or de-ionized water

  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

  1. Block membranes

  2. Incubate with Sample

  3. Incubate with Biotinylated Detection Antibody Cocktail

  4. Incubate with HRP-Conjugated Streptavidin

  5. Incubate with Detection Buffers

  6. Image with chemiluminescent imaging system

  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.